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Procedures Manual

New centres to join CABRI: Information authentication

New centres which are willing to join CABRI are requested to give information on their catalogues and databases for review.

New centres have to adhere to the Minimum Data Sets (MDS) and input processes (IP) defined by CABRI. Any comments and modifications proposed by the new centres to the MDS and IP will be examined by the Technical Committee that will amend MDS and IP accordingly if necessary.

New centres have to follow the guidelines for catalogue production, including flat file submission and import format.

Review:

  • Catalogue description (Subject covered, Total records)
  • Catalogue availability
  • Sample of records
  • Databases information:
    • on which system and software the database is running
    • update frequency
    • datafield description and brief authentication
Datafields:

Field name Name of the field in the local database
Description Detailed description of the contents and structure, when existing, of the field
Catalogue output Examples of the output of the catalogue
Authentication Authentication methods, including use of controlled vocabularies, agreed lists, reference papers and books, etc...

Minimum data sets

The Minimum Data Set (MDS) is the set of data that is asked to describe each product from each collection. Each catalogue field has to be filled in (with unknown or not applicable when no information is given).

The Recommended Data Set : collection centres are also asked to supply data for recommended fields; however, if data are not available, the product is still acceptable

Collection centres are free to provide data for other fields, which are part of the Full Data Set

A general MDS can be applied to each type of collections :

Identification

accession number, name

Origin

description, depositor, history..

Properties

 

Culture conditions

medium, subculture routines...

Restrictions

 

Hazard

 

Bibliography

Reference paper, links to other projects

 

For more details on MDS, input processes, flat file submission and import format, see the Guidelines for Catalogue Production.

 

Example:

Animal cell line catalogue ECACC

Description

The ECACC holds over 1500 cell lines representing 57 different species from exotic sources such as Iguana and red panda to the more common human and rodent. The diversity of the collection is further emphasised by the fact that the 600 human cell lines originate from 48 different tissues.

All cell lines from the collection are tested for absence of microbial contamination (mycoplasma, bacteria, fungi, yeasts) and authenticated by isoenzyme analysis and DNA fingerprint. As a result of funding from the EC BIOMED programme new and existing cancer cell lines in the collection (approximately 250) have been subjected to extensive literature searches resulting in detailed reports on 120 cell lines.

Catalogue availability

The catalogue is available CD-ROM (MAC and PC), hardcopy and via the internet (http://www.camr.org.uk).

For each cell line in the catalogue there is a full description and detailed culture conditions. For 120 of the cancer cell lines a report is also included with an extended bibliography and sections on cell characteristics and applications (electronic versions only). The electronic versions are fully searchable enabling all fields within each record can be searched e.g. "human chondrosarcoma" or "IL1 and TNF secreting monocytic cell lines"

Order and agreement forms can be edited and printed from the catalogue. Prices list is included.

 

Sample of records

ECACC number: 85081901
Name: 33B
Description: Rat nervous tissue oligodendroglioma
Culture medium: DMEM+2mM glutamine+10% FBS
Passage number: 55
Subculture routine: Split confluent cultures 1:3 to 1:6 ie seeding
at 2-4x10000 cells/cm2 using trypsin/EDTA;
5% CO2;37°C
Morphology: Neuronal
RCD No
Depositor Dr K Fields,Albert Einstein College ofMedicine,Bronx,New York USA
Reference Neuroscience 1985;15:877-885
Applications Neurobiological studies
Comments Ethyl nitrosourea induced tumour of rat nervous system.Derived from 17th in vivo passage of TR33B;a Ran-1 surface antigen positive line. The monoclonal antibody 217c can be used as marker for normal Schwann & rat glial tumour cells, binds to Ran-1

___________________________________________________________________________

BIOMED1 REPORT

The information below has been provided as a result of funding from the EC BIOMED programme. The information has been obtained from literature citing use of this particular cell line. The characteristics have NOT been verified experimentally by ECACC and, therefore, ECACC cannot be held responsible for failure of a cell line to comply to the characteristics listed below.

Cell characteristics:

1) expresses class II NGF receptor (low affinity),i.e. p75NGFR (1,2)
2) has no growth inhibitory activity (3)
3) exhibits phagocytic properties analogous tothose of normal Schwann cells (7,8,9,10)
4) Mycobacterium sp.phagocytosis.Not specific for Mycobacterium leprae (11)
5) binds to MAb 217c via Ran-1 antigen (12)

Applications:
1) Study of class II NGF receptor: binding properties in abscence of class I NGF receptor (2)
2) Screening drugs acting against Mycobacterium leprae: M.leprae is maintained within 33B cell line.(4)
3) Effect of anti-mycobacterial serum on uptake of Mycobacterium by 33B: comparison macrophage/33B cells interaction with Mycobacterium (7)
4) Mycobacterium phagocytosis: comparison with phagocytosis by macrophages (7,8,9,10)
5) Antibody production: can be used as an antigen (13)
6) Studies of regulation of myelin basic protein gene transcription in glial cells (5)

Bibliography:
(1) J Biol Chem 1992;267(32):22707-22710
(2) J Biol Chem 1992;267(20):13917-23
(3) Development 1991;112(1):33-42
(4) Antimicrob Agents Chemother 1991;35(7):1444-7
(5) Biochem Soc Trans 1991;19(2):85S
(6) Exp Cell Res 1989;182(1):173-85
(7) J Neuroimmunol 1987;14(2):235-9
(8) J Neuroimmunol 1986;13(1):109-13
(9) J Neurol Sci 1986;75(1):113-9
(10) Int J Leprosy Other Mycobact Dis 1986;54(2):294-9
(11) Int J Leprosy Other Mycobact Dis 1986;54(1):71-8
(12) Neuroscience 1985;15(3):877-85
(13) Prog Clin Biol Res 1977;15:179-90

___________________________________________________________________________

 

Databases information

Databases are maintained on MS-Access

Update:
database constantly
catalogue once a year
WEB monthly basis

Datafields:

Field name

Description

Catalogue

output

Authentication

TAG

field for database management

 

ECACC

AMEND_DATE

Date of amendements of the data

 

ECACC

ECACC_NO

ECACC unique identifier

X

ECACC

HAZARD

any hazard associated with the cell (ACDP category)

 

depositor, ECACC

NAME

name of the cell

X

depositor

SPECIES

origin of the cell

 

depositor, ECACC by isoenzyme,DNA fingerprint

TISSUE

origin of the cell

 

depositor

CANCER

origin of cancer

 

depositor

LEUK

origin of leukemia

 

depositor

DESC_KW

description of the cell

X

ECACC

MEDIUM

culture medium

X

depositor, ECACC by usage

PASSAGE_NO

passage number

X

depositor, ECACC by usage

SUB_ROUTE

culture methods

X

depositor, ECACC by usage

MORPH

morphology of the cell

X

ECACC

KARY

karyotype of the cell

X

depositor

RECEPTORS

receptors expressed by the cell

X

depositor

PRODUCTS

products of the cell

X

depositor

HISTO

histocompatibility type

X

depositor

RCD

deposit under the Research Council Deposit

X

ECACC under contract with MRC

DEPOSITOR

Name and address of the depositor

X

depositor

COUNTRY

country of the depositor

X

depositor

ORIGINATOR

depositor is the originator of the cell

 

depositor

REFERENCE

bibliographical references

X

depositor

REL_COND

conditions of release of the cell, restricted access and use

X

depositor

ATCC_DESIG

designation of the cell given by the ATCC

X

depositor, ECACC

PATENTS

cell under patent

X

depositor

COMMENTS

remarks

X

depositor ,ECACC

APPLIC

application where the cell can be used

X

depositor, ECACC from bibliography

PATHSCREEN

 

 

ECACC

BIOMED1

BIOMED1 report on cell characteristics in bibliography

X

ECACC from bibliography only, not verified by lab. experiments


Guidelines prepared for CABRI by CERDIC
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Site maintained by Paolo Romano. Last revised on February 2023.